Lactobacillus MRS Broth is a specially formulated growth medium used for the cultivation and enumeration of Lactobacillus species and other lactic acid bacteria. It provides the essential nutrients and optimal environment that support the growth of fastidious microorganisms commonly found in dairy, food, and probiotic samples.

What Is MRS Broth?

The term MRS stands for de Man, Rogosa, and Sharpe, the scientists who developed this medium. MRS Broth contains a balanced combination of peptones, dextrose, beef extract, yeast extract, polysorbate 80, sodium acetate, ammonium citrate, and magnesium sulfate. These ingredients provide nitrogen, carbon, vitamins, and minerals necessary for the rich growth of Lactobacillus.

This medium is designed for anaerobic and microaerophilic cultivation, making it ideal for isolating Lactobacillus, Streptococcus, and Pediococcus species.

Key Features of Lactobacillus MRS Broth

• Supports robust growth of lactic acid bacteria
• Suitable for both aerobic and anaerobic incubation
• High-quality, consistent, and contamination-free formulation
• Easy to prepare and use in microbiology laboratories
• Available in powder form for accurate measurement

Applications in Laboratory

Lactobacillus MRS Broth is widely used in food microbiology, probiotic research, and dairy product analysis. Researchers and students use it to:

• Cultivate Lactobacillus spp. from yogurt, milk, and fermented foods
• Perform viable count tests of lactic acid bacteria
• Conduct quality control in food and beverage industries
• Study bacterial metabolism and fermentation behavior

Because of its reliability, MRS Broth is a standard medium in microbiological research worldwide.

Preparation and Storage

To prepare the broth, dissolve 55 grams of MRS medium per liter of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the solution to room temperature before inoculating.
Store the dehydrated medium in a cool, dry place, tightly sealed to avoid moisture absorption.

Safety and Handling

Always follow proper laboratory safety protocols while handling culture media. Use gloves, a lab coat, and protective eyewear. Avoid inhaling powder or direct skin contact.

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Labtex Bangladesh provides:

• Laboratory-grade culture media and reagents
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• Excellent customer support

Whether you are a microbiology student or a professional researcher, you can trust Labtex Bangladesh for premium lab supplies and chemicals.

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