Mueller Hinton Agar (MHA) is a standard culture medium used in microbiology laboratories. It is best known for its role in antibiotic susceptibility testing, especially the Kirby-Bauer disk diffusion method. This medium supports the growth of most bacteria and provides consistent, reliable results.

The composition includes beef infusion, casein hydrolysate, and starch. These ingredients supply essential nutrients while the starch absorbs toxins that could affect antibiotic activity. This makes the medium ideal for sensitivity testing.

Mueller Hinton Agar is recommended by CLSI and EUCAST guidelines for antimicrobial testing. It is widely used in hospitals, diagnostic labs, research centers, and educational institutions. The medium is available in dehydrated powder and ready-to-use plates.

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