Mueller Hinton Agar (MHA) is one of the most commonly used culture media in microbiology. It is widely applied for antibiotic susceptibility testing (AST), especially the Kirby-Bauer disk diffusion method. The medium supports the growth of most non-fastidious organisms and provides reliable, reproducible results.

This agar contains beef infusion, casein hydrolysate, and starch. These ingredients supply essential nutrients for bacterial growth. The starch absorbs toxins released by bacteria, which helps prevent interference with antibiotic activity.

Mueller Hinton Agar is recommended by organisations such as CLSI and EUCAST for antimicrobial sensitivity testing. It is also used in clinical laboratories, research centres, and educational institutions. The medium comes in both dehydrated powder and ready-to-use plates.

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