Biuret Reagent 500mL Solution

The Biuret Reagent Solution (500mL) provides a reliable and accurate method for detecting and estimating proteins in laboratory samples. This reagent contains copper(II) sulfate, sodium hydroxide, and stabilizing agents, which react with peptide bonds to produce a characteristic violet or purple color in the presence of proteins. The intensity of the color directly correlates with protein concentration, making it a standard solution for qualitative and quantitative protein analysis.

Laboratories rely on Biuret Reagent for its stability, consistency, and reproducibility in biochemical and educational experiments. It plays a critical role in protein assays, clinical diagnostics, and food and agricultural testing. The 500mL volume ensures ample supply for routine laboratory use.

Key Features of the Biuret Reagent 500mL

• Type: Protein detection reagent (Biuret method)
• Volume: 500mL standard laboratory bottle
• Composition: Copper(II) sulfate solution with alkaline stabilizers
• Produces violet/purple color in the presence of proteins
• Ensures accurate, reproducible results in protein assays
• Suitable for qualitative and quantitative analysis
• Packaged for safe handling and long shelf life

Technical Information

• Principle: In alkaline conditions, copper(II) ions form a coordination complex with peptide bonds, producing a violet-colored compound.
• Detection Range: Effective for protein concentrations above 1 mg/mL
• Storage Conditions: Store at room temperature (15–25°C) in a tightly sealed container, away from direct sunlight
• Appearance: Blue liquid solution
• pH: Strongly alkaline (due to NaOH)

Applications of the Biuret Reagent 500mL

• Perform protein estimation in biochemical experiments
• Use in clinical diagnostics for serum and plasma protein analysis
• Conduct food quality control (measuring protein in milk, flour, and other samples)
• Apply in educational laboratories for teaching protein detection methods
• Support research in molecular biology, biochemistry, and nutrition

Safety & Handling

• Use standard laboratory safety precautions
• Avoid contact with skin and eyes; wear gloves and protective eyewear
• Dispose of according to local chemical waste regulations
• With Biuret Reagent Solution 500mL, you ensure precise protein detection and reliable results for educational, research, clinical, and industrial laboratories.