Hemocytometer Set

This hemocytometer is designed and used to count blood cells in the laboratory. Including one set of counting chambers, one set of blood-diluting pipettes for red corpuscles, one set of blood-diluting pipettes for white corpuscles, and one set of blood cover glass. Made in China.

A hemocytometer is also known as a Haemocytometer or a cell counting chamber. It is a tool used for manual cell counting. As the name implies, the hemocytometer was originally invented for quantifying blood cells.

A hemocytometer is a device used to count cells or particles in a liquid sample under a microscope. It was originally designed for blood cell counting but is now widely used in biology and medicine to count various types of cells, such as bacteria, yeast, or other cell cultures.

Structure of a Hemocytometer

• Counting Chambers: The hemocytometer consists of a thick glass slide with two mirrored, gridded chambers. Each chamber contains a specific volume of liquid, making it easier to calculate the number of cells per unit volume.
• Grid: The counting area is etched with a grid of squares, typically 9 large squares, each of which is subdivided into smaller squares. The most commonly used grid size is a 1 mm x 1 mm square, with a depth of 0.1 mm.

General Uses of Hemocytometer

• Cell Counting: To determine the concentration of cells in a sample.
• Viability Testing: By using stains like Trypan Blue, live and dead cells can be distinguished.
• Blood Cell Counts: For counting red and white blood cells in clinical settings.
• Microorganism Studies: For counting bacteria, yeast, or other small organisms in research.

Procedure for Use

1. Dilute Sample: Prepare the sample and, if necessary, dilute it to an appropriate concentration.
2. Load the Sample: Place a small amount of the liquid sample on the hemocytometer.
3. Microscope Observation: Use a microscope to observe and count the cells within the grid.
4. Calculate Concentration: Use the formula to calculate the concentration of cells in the original sample.

​The hemocytometer remains a key tool in many laboratories for simple and quick cell counting.