Mannitol Salt Agar is a selective and differential culture medium used in microbiology laboratories. It is designed to isolate and identify Staphylococcus species, especially Staphylococcus aureus.

The medium contains high salt concentration, which inhibits the growth of most other bacteria. It also contains mannitol and a pH indicator, allowing differentiation based on sugar fermentation. Colonies that ferment mannitol turn the medium yellow, while non-fermenters remain red or pink.

MSA is widely used in clinical labs, food testing laboratories, research centers, and educational institutions. It helps identify pathogenic bacteria and monitor hygiene in food or clinical samples.

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